Scientific Program

Day 1 :

  • Keynote Forum

Session Introduction

A. Pavlenko

Kiev Polytechnical Institute, Ukraine

Title: Possible ways to neutralize biopathogenic radiation.
Speaker
Biography:

A. Pavlenko has completed his PhD at the age of 37 years from Sevastopol Polytechnical Institute. He is the director of “Spinor International”, Head of the Laboratory of the   Electron Devices chair at Kiev Polytechnical Institute. i has published more than 50 papers in reputed journals and has been serving as Member of the editorial boards of a scientific periodical: Ukrainian Journal   Physical vacuum and Nature.

Abstract:

Techno pathogenic effect - a set of factors and processes that have any kind of negative effect or impact on living organisms resulting from scientific and technological progress of mankind.This is a summary of ideas and methods for protecting the public from the negative impact of electronic technology. While conducting research, the author tried to clearly define the prevailing negative impact on living of torsion fields (TF) in comparison with electromagnetic radiation. Electromagnetic fields warm the tissues of a person using all sorts of gadgets, while torsion fields affect the gene level. Much attention is paid to the technology of protection from  torsion fields.American biologists drew attention to this phenomenon, calling such radiation "nonclassical electromagnetic radiation". The specific absorption rate (SAR) is considered as an official standard for measuring the effects of electromagnetic radiation (EMR) from mobile phones on human brains.However, the biologists do not consider SAR measurements as an accurate representation of mobile phone EMR effects on the human brain in real life. As an alternative of SAR the increasing number of scientists uses the biological systems for evaluation of harmful effects of mobile phone EMR. In the above-mentioned works the positive moment is a reference to "non-classical" electromagnetic fields though their physical nature has not been disclosed. In our opinion the "non-classical" electromagnetic fields are torsion fieldsWe need to convey to people the truth about the impact of electronic technology on the living.

 

Speaker
Biography:

Amr Amin has completed his PhD at University of Illinois at Chicago and received a post-doctoral training in the field of molecular genetics at University of Pennsylvania School of Medicine. He started his academic career at UAE University where he serves now as a Full Professor of Cell & Molecular Biology. Amr’s research focuses on ways to control cancer, particularly liver cancer. He published many research articles, reviews, and serves as ad hoc reviewer and as an editorial member of many specialized peer-reviewed journals. He is also a member of many specialized societies and the sole recipient of many national and international scientific awards; Khalifa Award for Research being the most recent.

Abstract:

The multikinase inhibitor, sorafenib, remains for a decade now the first and only systemic therapy for the treatment of hepatocellular carcinoma (HCC). Thus, a novel approach against HCC is essential for a better therapeutic outcome. The aims of this talk is to assess the chemopreventive action of saffron’s main biomolecule, crocin, against chemically-induced liver cancer in rats, and to explore the mechanisms by which crocin employs its anti-tumor effects. We provide a network analysis of differentially expressed genes in tissues of animals pre-treated with crocin in comparison to induced-HCC animals’ tissues. To further support our results, we assessed the effects of crocin on HepG2 cells viability by treating them with various concentration of crocin; in addition, effects of crocin on cell cycle distribution of HepG2 cells were investigated. Network analysis identified NF-kB as a potential regulatory hub, and therefore, a candidate therapeutic drug target.

  • Sequence Analysis | Gene Expression | Simulation and Modelling | Health Informatics |Biomedical Informatics
Speaker
Biography:

Dr. Koltover, SciD (Biophysics), PhD (Chemical Physics), works in Institute of Problems of Chemical Physics, the premier organization of Russian Academy of Sciences, at the present time as chief researcher (principal investigator). Dr. Koltover has more than 250 scientific publications, including books and papers in the representative journals, presented plenary and oral presentations at scientific meetings in Austria, Canada, China, France, Greece, Italy, Israel, Japan, USA, Ukraine and Russia. As invited scientist (visiting professor), Dr. Koltover worked in Austria (Institute of Pharmacology and Toxicology, Vienna), India (Institute of Technology, Chennai, and Bhabha Atomic Center, Mumbai); Germany (Max-Planck Institute for Demographic Research, Rostock, and Institute for Physical Chemistry, Albert-Ludwig University, Freiburg), USA (National Lawrence Berkeley Laboratory, University of California, University of Pennsylvania, Philadelphia, and Northwestern University, Evanston). Main fields of experience: reliability (robustness) and aging of biological systems, free radicals and antioxidants, magnetic isotopes and nuclear spin catalysis, chemical bionics.

Abstract:

In engineering, reliability is defined as ability of a device to perform a preset function for a given time under given conditions. The foundations of mathematical theory of reliability were laid in 1950s due to the needs of aeronautic machinery, electronics, etc. Biological systems perform their functions in the presence of random factors which disturb all functional strata starting from the biomolecular level. Therefore, similarly to technical devices, they are not perfectly reliable, i.e. normal operations alternate with random malfunctions (failures). The field of systems biology, in dealing with the problem of reliability, incorporates investigations of failures, renewal processes, and elaboration of methods for testing bioreliability and so on. The regular conferences on bioreliability, starting from the first one in 1975, Kiev, Ukraine, have given the strong impetus to research in this direction. Not long ago, a new wave of similar research was spurred under the style of “biological robustness”. The main line of assuring the high systems reliability is prophylaxis or preventive maintenance, i.e. unreliable elements should be timely replaced for novel ones ahead the phase of their wear-out begins. The prophylaxis proceeds under the control of the functional elements of the highest level of hierarchy designated as the longevity-assurance networks (supervisors). The problem of reliability has direct bonds to aging and resistance of biological systems to deleterious environmental factors. From the reliability point of view, aging occurs as consequence of the genetically preset deficiency in reliability of biomolecular constructs while the mitochondrial free-radical redox-timer, located in the specialized neurons, serves as the stochastic mechanism of realization of the aging program. The longevity of human brain could reach 250 years should the antioxidant enzyme defense against the free-radical failures be perfect. Furthermore, the systems theory of reliability provides heuristic methodology for preventive medicine, including antioxidant therapy and anti-radiation defense.

Speaker
Biography:

Ali Fouladkar, 28 years old, a researcher in Big Data field, deals with the concept of Big Data, Weak Signal and Strategic Decision. Information System lecturer at Grenoble Graduate Business School (IAE Grenoble). The purpose of his thesis “Detection and Interpretation of weak signals from big data for a strategic decision : adoption of technology” is to identify which Communities of the Organizing Vision influence the decision of adoption a Big Data technologies to detect weak signals and support strategic decision.In the Bachelor and Master Degree level, he achieved the first rank in his university in the field of Business Management.Ali participated in many Research Days and Round Table related to Big Data issues in USA and France. His researchers presented and cited at high-level international conferences.

Abstract:

Big Data, the energy of the 21st century, the seed of innovative companies. The explosion of the volume of data and the emergence of Big Data drastically modify the decision-making process, particularly the strategic decision. Our research proposes a managerial approach to Big Data through qualitative studies based on the Organizational Vision.Our research analyzes the media and the scientific discourse surrounding Big Data with the objective to define an inter-organizational grid of the adoption of Big Data technologies used to detect weak signals and support the strategic decision. The objective of this research is to identify the Organizing Vision Communities that influence the decision of adoption of Big Data Technologies to detect Weak Signal from huge amount of data to help decision makers opting the best possible strategic decisions and to study how the discourse of the organizing vision influences the adoption decision of these technologies. Our research mainly investigates five sectors: health, financial markets, marketing, energy and security.Our research question is: Which communities of the organizing vision influence the decision of adoption of Big Data technologies to detect weak signals and support strategic decision?Our research based on three studies. The first analyzes the professional media discourse around the concept of Big Data, Weak Signal and Strategic Decision. The second analyzes the systematic literature review on Big Data concept, Weak Signal and Strategic Decision. The third study based on interviews with users, expert and professionals of Big Data, is interested in understanding how and why some Big Data technologies are adopted to detect weak signals and support strategic decision and not others.This research study the influence of different communities of organizing vision specifically on the decision of adoption of Big Data technologies and the contributions of these technologies to detect weak signals and support strategic decision.

Speaker
Biography:

Dr. Abdeen Mustafa Omer (BSc, MSc, PhD) is an Associate Researcher at Energy Research Institute (ERI). He obtained both his PhD degree in the Built Environment and Master of Philosophy degree in Renewable Energy Technologies from the University of Nottingham. He is qualified Mechanical Engineer with a proven track record within the water industry and renewable energy technologies. He has been graduated from University of El Menoufia, Egypt, BSc in Mechanical Engineering. His previous experience involved being a member of the research team at the National Council for Research/Energy Research Institute in Sudan and working director of research and development for National Water Equipment Manufacturing Co. Ltd., Sudan. He has been listed in the book WHO’S WHO in the World 2005, 2006, 2007 and 2010. He has published over 300 papers in peer-reviewed journals, 200 review articles, 15 books and 150 chapters in books.

Abstract:

Sudan is an agricultural country with fertile land, plenty of water resources, livestock, forestry resources, and agricultural residues. Energy sources are divided into two main types; conventional energy (woody biomass, petroleum products, and electricity); and non-conventional energy (solar, wind, hydro, etc.). Sudan possesses a relatively high abundance of sunshine, solar radiation, moderate wind speeds, hydro, and biomass energy resources. Like many tropical countries, Sudan has ample biomass resources that can be efficiently exploited in a manner that is both profitable and sustainable. Fuel-wood farming offers cost-effective and environmentally friendly energy solutions for Sudan, with the added benefit of providing sustainable livelihoods in rural areas. Biogas from biomass appears to have potential as an alternative energy in Sudan, which is potentially rich in biomass resources. This is an overview of some salient points and perspectives of biomass technology in Sudan. This current literature is reviewed regarding the ecological, social, cultural and economic impacts of biomass technology. This chapter provides an overview of biomass energy activities and highlights future plans concerning optimum technical and economical utilisation of biomass energy available in Sudan. It is concluded that renewable environmentally friendly energy must be encouraged, promoted, implemented, and demonstrated by full-scale plan especially for use in remote rural areas.

Speaker
Biography:

Abstract:

The order of genes (i.e. physical location of genes) within a eukaryotic genome is not random, rather they are because of several different kinds of evolutionary genomic rearrangements such as insertion and deletion etc. Several studies in the past – which focused  on a few examples – have shown that a set of genes involved within a biological function are co-expressed and co-clustered together within the genome . In humans, some genes from the Purine metabolism pathway such as PAICS (GeneID 10606, encoding amidophosphoribosyltransferase EC 2.4.2.14) and PPAT (GeneID 5471, encoding SAICAR synthetase EC 6.3.2.6) are co-clustered in the genome at location 4q12, whereas other genes from the same pathway are located in different positions . My research focuses on examining the evolutionary association between gene order and biological functions. More specifically, I am interested in addressing the following fundamental research questions: a) How are co-clustered genes functionally and evolutionarily related? b) Why do genes from only a specific set of biological functions (and how many of them?) are grouped together within the human genome, and c) How do they affect metabolism, on a genome-scale. The gene-function association will be taken from Molecular Signatures Database (MSigDB), which has annotations from a range of databases. Genomic location from the human genome, and genomic functional association will be used to reconstruct a functionally co-clustered gene network. The network will be divided into topological modules and their functional association will be examined. The differential expressions will be studied using limma package in R. For a pair of genes, the differential expression pattern across conditions will be examined and, using statistical analysis, a gene co-expression network will be constructed. The resulting network will be used to address the objectives of the study.

Speaker
Biography:

Abstract:

Since the first reported outbreak of dengue hemorrhagic fever in Pakistan, several mini outbreaks have been observed in the country. In Pakistan, Serotype 2 (DEN-2) is documented since 1994 and is the major circulating serotype in the region. Dengue virus serotype 3 (DEN-3) was first reported in Karachi during 2005 outbreak. Dengue virus has now become a serious threat to humans especially in developing countries. As yet, no vaccine and proper treatment is available for dengue virus, so control of vector is the only option left to control dengue infection. Aedes mosquitoes are well known vector for this virus but some studies show that dengue virus can also survive in Culex mosquitoes. So, we have conducted a study to compare the vectorial role of Aedes and Culex in Lahore by using molecular techniques such as PCR and nucleotide sequencing of the C-prM gene junction of Dengue virus.A total of 1,110 adult resting mosquitoes were collected from selected localities of Urban Lahore. Of collected Aedes (n=498) and Culex (n=612) each were divided into 45 pools separately. After molecular analysis, 6 pools of Aedes (13.33%) and 9 pools of Culex (20%) were found positive for dengue virus. Interestingly, in all positive pools only serotype-2 of dengue virus was detected.We have found only serotype-2 of dengue virus in our study but other studies also report the presence of serotype-3. The occurrence of serotype-1 and serotype-4 in Pakistan, till now, is not reported. The presence of dengue virus in Culex in such a high per cent is alarming.

Speaker
Biography:

Segundo Mesa Castillo. As Specialist in Neurology, he worked for 10 years in the Institute of Neurology of Havana, Cuba.  He has worked in Electron Microscopic Studies on Schizophrenia for 32 years. He was awarded with the International Price of the Stanley Foundation Award Program and for the Professional Committee to work as a fellowship position in the Laboratory of the Central Nervous System Studies, National Institute of Neurological Diseases and Stroke under Dr. Joseph Gibbs for a period of 6 months, National Institute of Health, Bethesda, Maryland, Washington D.C. USA, June 5, 1990. At present he is member of the Scientific Board of the Psychiatric Hospital of Havana and give lectures to residents in psychiatry.

Abstract:

There is increasing evidences that favor the prenatal beginning of schizophrenia. These evidences point toward intra-uterine environmental factors that act specifically during the second pregnancy trimester producing a direct damage of the brain of the fetus [1]. The current available technology doesn't allow observing what is happening at cellular level since the human brain is not exposed  to a direct analysis in that stage of the life in subjects at high risk of developing schizophrenia. Methods. In 1977 we began a direct electron microscopic research of the brain of fetuses at high risk from schizophrenic mothers in order to finding differences at cellular level in relation to controls. Results. In these studies we have observed within the nuclei of neurons the presence of complete and incomplete viral particles that reacted in positive form with antibodies to herpes simplex hominis type I [HSV1] virus, and mitochondria alterations [2]. Conclusion. The importance of these findings can have practical applications in the prevention of the illness keeping in mind its direct relation to the aetiology and physiopathology of schizophrenia. A study of the gametes or the amniotic fluid cells in women at risk of having a schizophrenic offspring is considered. Of being observed the same alterations that those observed previously in the cells of the brain of the studied foetuses, it would intend to these women in risk of having a schizophrenia descendant, previous information of the results, the voluntary medical interruption of the pregnancy or an early anti HSV1 viral treatment as preventive measure of the later development of the illness.

Speaker
Biography:

Abstract:

Cytotoxic lymphocytes (CL) eliminate virus infected and tumour cells by initiating target cell apoptosis through two pathways: ligation of cell surface death receptors and the granule exocytosis pathway. The granule exocytosis pathway involves secretion of cytotoxic granules containing perforin and granzymes into target cells, thereby initiating apoptotic pathways. Perforin is a pore forming protein that utilizes a poorly understood mechanism to efficiently deliver granzymes, a family of serine proteases synthesized by CL, into the target cell cytoplasm.In humans, there are five Granzyme genes (GzmA, GzmB, GzmH, GzmK, GzmM) located at three different chromosomal loci. Based on their specificities theses serine proteases can be grouped into tryptases (GzmA and GzmK), aspases (GzmB) and chymases (GzmM and GzmH). These different granzymes perform intra– and extracellular functions. Three intracellular roles, dependent on perforin-mediated delivery into target cells, have been described: cytotoxicity, accessory functions and cytokine signalling. Extracellular functions, independent of perforin, have been reported for some granzymes and involve cleavage of extracellular matrix; possibly to facilitate immune cells migration. Although less data is available for these proteases, however the over-expression of these genes in certain disease conditions like autoimmune diseases has made it a subject of interest for designing potential inhibitors to stop their activity, as when they are synthesized in excess, becomes lethal to normal cells in a human body. This presentation will highlight the significance of these proteases and their possible involvement in various disease conditions.

Speaker
Biography:

Abstract:

Current technology and social media play a big role in modern life. Also, people are getting more involved with their health information. Where medical advices should meet social legal requirement with the respect to patient rights and needs. Current healthcare systems are overloaded with increasing flow of patients. In case of chronic patients or in time of seasonal infections or operating simple surgical procedures, the doctors and the assistant team can be faced with huge number of patients and they have to spend log time in clinic to serve them.We provide a professional Medical Social Media, our network doctors have their own cloud clinic an advanced tele-medicine system based on lightweight set of medical devices connected to a mobile based application to connect the patient and doctor or doctor to hospital were all medical measures are available to the remote doctor for efficient medical support. A layout of social media platforms and its daily use will help to change the lifestyle of the patient and that will reflect on the public health.  Our solution is more than mobile based appointment and Video/Audio communication system. It collects patient data from the devices to the mobile App and from that to our cloud-based IT system. Integration of Telemedicine Hardware and Software Components will definitely lead to a better telemedicine practice when the data can be shared with the doctor in real time at any time. The system powered by artificial intelligent to analyzes the medical data (bio-test, visits reports, genetic testing), activities and raises certain alarms when certain abnormalities accrue. We believe our application will change the way medicine is practiced as well as adding a great trustful resource of getting medical advices or real time consultations. It will bring advances in IT, mobile computing, body sensors, and cloud computing to the medical practice and will create a new form of electronic health records and more effective deliverance of healthcare services. All this is integrated in a professional social media platform, which will create an impact on the public health after the remarkable lifestyle change of the society with an equal opportunity of treatment and healthcare to everyone with an Internet connection. Thus, easy healthcare access will help people to manage both illness and health.

Day 2 :

  • Keynote Forum

Session Introduction

Sergey V. Petoukhov

Russian Academy of Sciences, Russia

Title: Quantum-Information Genetics and New Symmetries in Long DNA-Texts
Speaker
Biography:

Head of Laboratory of bio mechanical systems research in Mechanical Engineering Research Institute of the Russian Academy of Sciences; Chief researcher of the Center of interdisciplinary researches of musical creativity of the Tchaikovsky Moscow State Conservatory; Editor-in-Chief of International Journal of Mathematical Sciences and Computing. Russian organizer of the series of annual Russian-Chinese conferences Artificial Intelligence, Medical Engineering, Education. Co-organizer of many conferences in different countries. He is the author of 7 books and more than 200 other works. He graduated from the Moscow Physical-Technical Institute in the Department of Physics of Living Systems.

Abstract:

Hidden symmetries in long sequences of oligonucleotides of single stranded DNA from their representative set are described. These symmetries are an addition to symmetries described by the second Chargaff’s parity rule (%A ≅ %T and %G ≅ %C). These new symmetries and their rules concern collective probabilities of oligonucleotides from special tetra-alphabetical sets in long DNA-texts including all chromosomes of human and some model organisms [1]. These rules of tetra-alphabetical probabilities are considered as candidacies for the role of universal rules of long DNA-sequences. On the basis of the known quantum-mechanic statement that quantum state of a multi-component system is defined by the tensor product of quantum states of its subsystems, a quantum-information model of genetic symmetries of these collective probabilities was proposed. In this model, nitrogenous bases C, T, G, A of DNA were represented as computational basis states of 2-qubit quantum systems [1]. The biological meaning of the new quantum-information symmetries of long DNA-texts can be connected with the common ability of organisms to grow on the basis of incorporation into their body of new molecules of nutrients becoming new quantum-mechanic subsystems of the united quantummechanical organism.

Speaker
Biography:

Dr. Francis Amirtharaj serves as an Assistant Professor/Research Scientist at the Thumbay Research Institute for Precision Medicine, Gulf Medical University, Ajman, UAE since 2016. He earned his Ph.D. from the Madras University/CCMB and He has conducted research at Centre for Cellular and Molecular Biology, Hyderabad, India as a post-doctoral fellow.  He is expertise in various molecular biology techniques, such as next-generation sequencing (NGS), Sanger sequencing, real-time PCR etc. His current research interest to study the influence of p53 and its inactivation mutations in various cancer. He has ample teaching experience in Molecular Biology.

Abstract:

Dysferlin (DYSF) is a 237kDa transmembrane protein found in sarcolemma, known to facilitate membrane repair in skeletal muscles, muscle differentiation, membrane fusion, etc. The deficiency of DYSF due to mutations is one of the cause for Limb-Girdle Muscular Dystrophy 2B (LGMD2B), Miyoshi Myopathy (MM) and a distal anterior compartment myopathy (DMAT), which are collectively named dysferlinopathy. DYSF gene is located on chromosome 2 (2p13), encompasses 55 coding exons and spans 150 kb of genomic DNA. To evaluate the role of DYSF gene mutation(s) in Indian dysferlinopathies, we have performed both genetic and immunological analyses in 66 clinically defined cases. Western blot analysis of muscle specimens confirmed that all of them had dysferlin deficiency. All the 55 exons of DYSF were sequenced to find mutation(s) that are associated with dysferlinopathies. Sequence analysis of the dysferlin gene in these patients revealed a total of 84 variants. Of these, 51 were novel mutations that include 6 missense mutations; 6 silent mutations; 1 termination mutation; 3 frameshift mutations; 7 splice site variations; and 28 in intronic region and 33 previously reported mutations (5 missense; 7 silent; 1 termination mutation; 5 splice site; and 15 intronic region). This is the first extensive study of DYSF mutations in Indian dysferlinopathy samples. We have identified several novel and functionally significant mutation, which can be used as diagnostic markers.

Speaker
Biography:

Prof. Petr Mikhailovich Korolev (PMK) is a President of Studia Korolevae Int. Kudymkar Russia. PMK has 45 years of research, teaching and administrative experiences. He has taught Mathematics, Philosophy, Debating, Computer science and Methodology. PMK lectured at Turku, Finland; Tartu, Estonia; Hesbjerg, Denmark; Odessa, Ukraine; Aktubinsk, Kasakhstan; Minsk, Belarus; Cloverdale, AL, USA, and other places. Universities of Nizhny Novgorod, Saint Petersburg, Belgorod, Perm, Izhevsk, Odense, and Kudymkar (Udmurt state University) were the PMK workplaces (research). PhD on radiophisics, DSc on System Methodology.PMK took part in different research projects: quantum chemistry 1976, quantum radiophysics 1977; applied pedagogics 1979-1986; descrete mathematics and mathematical statistics 1980-1985; philosophy of P.A.Florensky 1984-1988; ethnology 1995-2008; sociology and philosophy of science 2003-2017; and others. PMK is a member of Editorial Boards, Scientific committees and Advisory councils in several International Journals, Assotiations, Societies and Institutions. Member of WFSF, advisor at American Biographical Institute, stakeholder at World GMN, member of Strategical Initiative agency (Leader ID), Russia, active member of Moscow methodology circle (movement). Doctor honoris causa at Hesbjerg peace reseacrh colledge, DK.

Abstract:

The aim of the work is to develop the core theory of interaction of the processes, such as problematization, schematization, objectification, positioning. These processes are the constructs of the technology of organization and management of thought processes. As a focus, we chose what makes the group holistic and cohesive. Taking as a basis the experience of discussing these processes and their interactions, the authors develop two versions of the theory, introducing methods of combinatorics and discrete mathematics into the constructive field. The metric basis of the core theory of interaction leading to cohesion is constructed, hypotheses of symmetry of processes are put forward, laws of interaction are offered. In the article, research restrictions related to the enneadic metric are adopted, the method of analogy with the core theory of modern physics of F.Wilczek is used. An application is presented in the form of a game with representatives of the value categories that make up the corporate philosophy of Bio Informatics. The results of the article are of practical use in the development of the microbiology The proposed theory can be applied to the development of an optimal set of tools for gene ontology, taking such tools as AMIGO, OBO-EDIT as the analyzed material. Game simulation and modelling can also be improved by applying cohesion theory. An analysis of the quantitative understanding of the biological system, as well as the possibility of predicting systemic features that are the goal of system biology, and the field of its application, related to the consideration of nonlinearity, may also include a theory of cohesion. In pharmaceuticals and the development of drugs in terms of their effects on the body, the effect of cohesion is importanThe architecture of the core theory is original, the theory is important for programming and controlling life processes.

  • Gene Ontology | System Biology | Metabolic Pathways | Genetic Regulatory Networks
Speaker
Biography:

Dr Nouman Rasool has completed his doctorate in Biochemistry from the University of Punjab. Currently, he is working as Chairman, Department of Life Sciences at University of Management and Technology, Lahore. Furthermore, he has been working as a visiting faculty member at Dr Panjwani Center for Molecular Medicine & Drug Research, University of Karachi. He has 16 research publications in renowned international journals and his area of research is Forensics Serology, Forensic DNA analysis, Protein Chemistry, Enzymology, Drug Discovery and Computational Biology.

Abstract:

Alphaviruses, which belong to the family Togaviridae, are single-stranded positive-sense RNA viruses and contain various viruses. Two major viruses which have more economic, medical and theoretical importance are Chikungunya virus (CHIKV) and Sindbis viruses (SINV). These viruses are fundamentally known to cause arthritic disease and encephalitis in humans and are grooming as a potential threat to worldwide nations. Individual development of drugs against each virus can be costly and resource consuming, thus, there is a dire need for the identification of such potential inhibitors, which can inhibit the alphaviruses, mutually. Based on this issue, the present study targets the identification of inhibitors against two major members of Alphavirus, Chikungunya virus (CHIKV) and Sindbis viruses (SINV). A total of 2750 phytochemicals from various medicinal plants are docked against non-structural proteins (NSPs) of CHIKV and SINV. The ADMET test was performed to determine various pharmacological parameters of the phytochemicals. The docking was performed using Auto Dock Vina. Based on this, mutually inhibiting compounds against CHIKV and SINV are screened and density functional theory (DFT) based analysis was carried out on ORCA to analyze the reactivity of the inhibitors at the binding pocket of the proteins. Hybrid exchange-correlation was applied for quantum mechanistic calculations to predict molecular orbital energy descriptors. Out of 2750 phytochemicals from various medicinal plants, 108 chemicals have passed ADMET test and Lipinski’s rule criteria showing that these are safe for human use. On docking with proteins, 108 phytochemicals have shown highest different binding affinities with the viral protein. Reactivity of the compounds was analyzed using the B3LYP correlation of DFT, and from all these compounds, only those compounds are screened which show strong binding affinities against NSPs and are mutual in CHIKV and SINV. Based upon the results, it is concluded that the screened phytochemicals are highly competent to impede the replication of two alphaviruses i.e. CHIKV and SINV. These compounds are safe for human use and are the ideal candidates to cure an infection caused by CHIKV and SINV in humans.

Day 3 :