Sequence assembly refers to aligning and merging fragments from longer DNA sequence in order to reconstruct the original sequence. The longer sequence resulted from sequence assembly is called as a contig sequence. During sequence assembly the short DNA fragments may also be aligned to a reference sequence in order to see the difference between the contig sequence obtained from the reference sequence. Sequence assembly identifies the intrinsic features of the sequence such as active sites, post translational modification sites, gene-structure, distributors of introns and exons. Identification of sequence difference and variations such as point mutation and single nucleotide polymorphism in order to get the genetic markers.
- Track 1-1 DNA Sequencing
- Track 2-2 Sanger sequencing method
- Track 3-3 Shotgun sequencing method
- Track 4-4 Gene sequence determination
- Track 5-5 Expressed sequence tags